hi all.. when i plot a calibration curve for reverse phase method response.. i.e. amount injected vs. total area obtained for a protein.. the curve does not look linear.
ie. the slope for say 0.2-4% solution of 15 microgram injection (100%) is different from that of 10-120% of 15 micrograms. when plotted in the same curve ie. 0.2-120% the lower points near 0.2% show 200% recovery based on the amount calculation from the slope and intercept values obtained for this curve.
i tried plotting a log amount-log area curve.. and this fits well with desired accuracy.
is this common behavior of protein in such a method. is such behavior acceptable?
also in such cases how would %impurities be quantitated. would i have to inject 3-5 amounts, plot a log-log curve every time and calculate %impurities for the test sample/ is this common practice..
or is area% reporting okay.
also, how to report % purity.
thanks!
0
No replies to this topic
