Posted 13 June 2009 - 11:54 AM
I'm currently working on hepatocellular carcinoma cells (HuH-7); testing the effect of an anticancer drug..
but I'm looking for a positive control to compare my results with...
Please tell me if you know any! (favorably affecting the p53 expression in p53-transfected cells.)
Posted 14 June 2009 - 11:14 AM
cytotoxic drugs like doxorubicin is fine
Thanks yobou, but doxorubicin acts through inhibiting topoisomerase enzyme and inhibiting DNA unwinding...
but i'm looking for a pro-apoptotic drug that acts through up-regulation of p53 tumor suppressor gene..
Posted 14 June 2009 - 08:42 PM
The relationship between DNA damge, G2-M arrest and activation of p53 is very well documented. if you write (doxorubicin-induced p53 activation ) on the pubmed or google you will get many reports
Edited by yobou, 14 June 2009 - 08:52 PM.