Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

DRG MARKER


  • Please log in to reply
1 reply to this topic

#1 iceman08

iceman08

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 12 June 2009 - 10:46 AM





I was hoping someone could help me with the following:

1) An antibody that is specific to dorsal root ganglion (DRG) cells. I am trying to decipher in my primary DRG culture which cells are DRG cells and which are not (i.e., schwann cells)

Moreover, if anyone has a thorough protocol of culturing primary DRGs could you please send it.


Thank you.
[/size]


#2 gfischer

gfischer

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 195 posts
9
Neutral

Posted 15 June 2009 - 06:32 AM

I assume you mean you want to identify which cells are neurons in your culture. If so, the best Ab to use would be NeuN, which stains all neuronal nuclei. For glia, I'd recommend GFAP.

A good standard protocol for DRG culture is:
Nat Protoc. 2007;2(1):152-60

I've also attached the protocol I use, which is a modified version of the Malin protocol.

It may take a few tries to get it right, but keep at it.

Good luck

Attached Files


Above all things, if kindness is your king,
then heaven will be yours, before you meet your end




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.