Questions about 260/230 when reading DNA conc.
Posted 12 June 2009 - 09:29 AM
Posted 12 June 2009 - 09:51 AM
I'm a summer student working in a lab and I've been doing a fair amount of DNA extraction using quigen's DNA extraction microkit. I've been using a Nanodrop to do my concentration readings and while my 260/280 ratios are usually above 1.8, my 260/230 ratios are pretty low-- usually below 0.5. What does that say about my DNA? Can it affect doing PCR on those samples? Any advice on how to clean it up?