I've cloned and transfected my cells with a full length gene construct (only the CDS). Now I'm looking to order antibodies agaisnt my protein. Most antibodies for my protein are polyclonal and target the the C-terminal. I don't have experience in ordering antibodies, this is my firsat time. Should I buyteh ones targeting the N terminal?
If I adda tag to my constrcut, will taht affect the antibodie sthat target eth c-terminal?
Submit your paper to J Biol Methods today!
1 reply to this topic