I would like to make a North-Western Blot to detect RNA-protein interaction.
Is there something special I have to consider?
Or can I simply use a standard protocol for Western Blot hybridization and detection?
I am making a vacuum RNA blot on a nitrocellulose membrane. I would then incubate the fixed membrane with cell extract and afterwards start the standard protocol with my first antibody...
Thanks in advance!
Edited by mastermi, 11 June 2009 - 06:01 AM.