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Can you use a single channel on a two color array?

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#1 Claes



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Posted 11 June 2009 - 05:23 AM

Hello everyone,
I was wondering if it is possible to use one of the channels for a two color array like you use a "normal" one-color array (e.g. an Affy chip or a filter) after you have already hybridized both samples? Or is the data from one channel dependent on the other channel after both are hybridized? I.e. is the amount of probe on the slide generally limiting (competitive binding) or are there other problems like spillover or quenching or something?
We have used a two-color array but not I want to use only one of the channels...
Thanks in advance!

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