What is stringent vector?
Posted 11 June 2009 - 05:21 AM
What are the examples? Is there any reading material available on this? I googled, but could not find proper information.
Posted 11 June 2009 - 04:45 PM
There are many vectors, some use T7 promoter, some lac, T7/lac etc... Each promoter has different stringency. T7 is known to be leaky, so some vector will include a lacI to prevent the leakage, some will have pLysS etc.
Posted 12 June 2009 - 01:12 AM
Yes. Its for expression in E coli
So the adjective 'stringent' before vector is always related to the promoter of the vector..right?
As you said, expression systems consisting of T7 promoter are considered to be leaky, meaning they are not stringent. Why pET vectors (containing T7 promoter) are considered stringent? Can you comment anything on 'stringency' of tet promoter systems (eg. pASK-IBA7) ? If I am getting very low level of expression with pASK-IBA7 vector, does it mean that I may get good levels in pET since this is not a stringent vector (or is it?) CONFUSED! What are other non-stringent vectors?
The link that u posted is not opening