4 replies to this topic

[ragno]

I usually use Linear polyacrylamide as a carrier for DNA precipitation by EtOH. I have read about using glycogen as a carrier, I would like to know if anyone knows a method avoiding not to use acrylamide (for which legislation is hard in France) as carrier for DNA.

Thanks for all

[mariet]

Hello,

I know for the french legislation, i work in french laboratory. As carrier you can use glycogen at 10 micro or plus when you precipitate with EtOH. You can also put microtube at -80°C 15mn or overnight at -20°C when small amount DNA. Glycogen is put into tube before Na acetate+EtOH. You can also use yeast tRNA as carrier, it's work well.

michel

[anonymous]

Are you sure you are not confusing POLYacrylamide and acrylamide (the monomer). LPA (Linear Poly Acrylamide) can be purchased cheaply from Sigma and used as a carrier.

[anonymous]

You can also use dextran blue.

[mariet]

I have searced in Sigma, but I have not found LPA.Where could I find it (is it solid or liquid)?Thank you for all