I am carrying MDA-MB-157 cells (freshly from ATCC) and found they growed very very slowly. I just followed some groups to use the medium of DMEM+10%FBS and culture them in 5% CO2 + 95% air incubator.
Anyone knows what medium can be used and help the cells grow faster? Appreciate for any useful information. Thank you.
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#2
bob1
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Posted 11 June 2009 - 05:07 PM
ATCC said:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0°C
Atmosphere: air, 100%
Temperature: 37.0°C
#3
Jerry
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Posted 12 June 2009 - 06:23 AM
It makes sense. Thank you very much, Bob1.
As you got them from the ATCC, they will have been grown in the above medium... to use DMEM you need to wean them on to it slowly over several passages by using different mixtures of the two media.
bob1, on Jun 11 2009, 09:07 PM, said:
ATCC said:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 37.0°C
Atmosphere: air, 100%
Temperature: 37.0°C
