Hi everyone
Does anybody have the experience in preparing folic acid solution for the cell culture
I purchased the powder from Sigma and according to their instructions, the powder should be dissolved in 1M NaOH
Although the final dilution is 1:50,000 in tissue culture medium, it still seems risky to me to add such a basic solution to the medium
I'll appreciate any help
Thanks a lot
Michael
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2 replies to this topic
#2
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Hmmm, I think it's working
Posted 11 June 2009 - 05:19 PM
Media are buffered, adding one part in 50,000 should not drastically change the pH even if it was 10 M NaOH.
#3
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Posted 12 June 2009 - 11:49 PM
bob1, on Jun 12 2009, 04:19 AM, said:
Media are buffered, adding one part in 50,000 should not drastically change the pH even if it was 10 M NaOH.
Thank You very much
Indeed, I've added one part in 50,000 to the medium and no pH change was observed.
Best Regards
Michael
