Folic acid solution for cell culture
Posted 10 June 2009 - 12:07 PM
Does anybody have the experience in preparing folic acid solution for the cell culture
I purchased the powder from Sigma and according to their instructions, the powder should be dissolved in 1M NaOH
Although the final dilution is 1:50,000 in tissue culture medium, it still seems risky to me to add such a basic solution to the medium
I'll appreciate any help
Thanks a lot
Posted 11 June 2009 - 05:19 PM
Posted 12 June 2009 - 11:49 PM
Media are buffered, adding one part in 50,000 should not drastically change the pH even if it was 10 M NaOH.
Thank You very much
Indeed, I've added one part in 50,000 to the medium and no pH change was observed.