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Folic acid solution for cell culture


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#1 Michaelro

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Posted 10 June 2009 - 12:07 PM

Hi everyone
Does anybody have the experience in preparing folic acid solution for the cell culture
I purchased the powder from Sigma and according to their instructions, the powder should be dissolved in 1M NaOH
Although the final dilution is 1:50,000 in tissue culture medium, it still seems risky to me to add such a basic solution to the medium
I'll appreciate any help
Thanks a lot
Michael

#2 bob1

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Posted 11 June 2009 - 05:19 PM

Media are buffered, adding one part in 50,000 should not drastically change the pH even if it was 10 M NaOH.

#3 Michaelro

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Posted 12 June 2009 - 11:49 PM

Media are buffered, adding one part in 50,000 should not drastically change the pH even if it was 10 M NaOH.


Thank You very much
Indeed, I've added one part in 50,000 to the medium and no pH change was observed.
Best Regards
Michael




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