White speckles on western blots
Posted 10 June 2009 - 07:57 AM
Both these images are pretty small (the best I could do with our scanner) but if you zoom in (Ctrl + Scroll wheel on mouse) you can see the problem.
My supervisor has less experience than I do, and the woman-of-experience in the lab across the hall has no suggestions. Any clue?
Posted 10 June 2009 - 10:42 AM
Or is it possible that the pouch you incubate your membrane in with the antibody got stuck onto the membrane at that specific spot, therefore preventing the ab to bind?
Posted 11 June 2009 - 03:08 AM
Posted 11 June 2009 - 05:50 AM
Or did you accidentally grab on the membrane ? I have also seen speckles like that after a student grabbed on the membrane...