Also, will all DH5alpha transformants on a given plate yield the same amount of plasmid DNA? If not, what factors affect the ultimate yields.
Finally, some references suggest that it is wise to use a higher antibiotic concentration to select for plasmid containing host cells then is necessary. For instance, I have seen one paper that suggests a Kanamycin concentration of 100 mg/L as opposed to 50 mg/L. It seems logical that this would help select for cells with more plasmids but does anyone know if this is actually the case? Perhaps it is more logical to think that an Ampicillin plasmid would work better in this model considering the proteinaceous resistance mechanism.
Thank you very much for your time. Any thoughts on this subject are greatly appreciated.