Non-specific bands on EMSA
Posted 09 June 2009 - 12:52 PM
I am not sure where my problems lie - I think my probes are annealing and labeling okay. Any ideas or suggestions would be gratefully received.
Posted 12 June 2009 - 11:17 AM
need a bit more info. are you using DS DNA as your labeled probes? are they annealed oligos? PCr productss?
these sound like non-specific bands to me. you can also try dIdC or dGdC as your competitors. salmon sperm DNA can degrade over time and give odd results.
Posted 15 June 2009 - 10:04 AM
Thanks for giving this some thought,