hi admin and chatter,
today, i'm using running re to cut my sequence, yes the re was cutting my sequence, my problem is the re was cutting at wrong site. base on http://tools.neb.com...tter2/index.php, b4 i order the RE, the re should cutting 123, 225 (example). but now base on my gel picture ..the RE not cutting what we aspect in NEB.. i'm using 100 bp marker.
pls give some opinion if u had same problem.. some solutions... thank you
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4 replies to this topic
#2
mdfenko
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Posted 09 June 2009 - 12:36 PM
you may be seeing star activity.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
#3
swanny
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Posted 09 June 2009 - 05:54 PM
can we see the gel image please? And what enzyme you're using.
Heart disease kills more women than breast cancer, but heart attack symptoms differ from men's symptoms. Get to know your heart... it could save your life.
#4
zack
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Posted 10 June 2009 - 02:08 AM
more detail, actually i'm doing RE digestion of PCR products.
#5
mdfenko
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Posted 10 June 2009 - 07:03 AM
you can look at a similar (same?) problem's responses in the biotechniques forum.
pay particular attention to the response made by onyourcase.
pay particular attention to the response made by onyourcase.
talent does what it can
genius does what it must
i do what i get paid to do
genius does what it must
i do what i get paid to do
