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RNase and PCR


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1 reply to this topic

#1 Helena Lorenzo

Helena Lorenzo

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Posted 20 February 2002 - 06:54 AM

Hi, guys
I want to clean my DNa preparations to do PCr with them. I have heard RNase can inhibit somehow the Taq polymerase. Do you have an idea about it?
How can I prepare RNase solution?

Thank you very much


#2 TINA JAMES

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Posted 27 February 2002 - 04:57 AM

I am extracting plant DNA and the protocol adds in Rnase in the extraction buffer stage.use 1ugm of Rnase A per ul buffer with a 10 minute incubation @37 degrees after adding extraction buffer then continue preps.




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