New to shRNA
Posted 08 June 2009 - 12:42 PM
Posted 08 June 2009 - 11:02 PM
Posted 09 June 2009 - 10:14 AM
Reporter asaay could be an option to screen potent shRNA in 96-well before making stable lines. Also, the reporter asaay can be carried out in 293T cells, the most transfectable cell lines, in case your model cells are not very transfectable.
Hope it helps.
Posted 18 June 2009 - 07:17 AM
Posted 22 June 2009 - 06:49 AM
Sigma does carry TRC clones. Not all are validated, but many are. Validation data is viewable when you log into the website.
I work with a big set of TRCN vectors as well and validated them in 96W on western.
You don't need more lysate than you can load on a gel anyway and a confluent 96W lysed with 50uL sample buffer is really more than you need.