No replies to this topic

[medivh]

i have been doing a clone which is supposed to replicate in staphylococcus. carnosus.
with electroporation, i could achieve an ideal transforming efficiency with pure DNA, but there lies a problem: there are false positive colonies on B2 broth with kanamycin of a concentration of 50ug/ml.
any one knows an ideal concentration? or how should i determine one?
i have run tests on that, but low to 25ug/ml would stop wild type s. carnosus from growing.
contaminations? i don't know......