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RT-PCR


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#1 bzhou

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Posted 03 January 2002 - 10:22 PM

My RT-PCR worked a few months ago, but now it doesn't work. The more strange thing is there is a right-size band in no-RT although the RNA is treated with DNaseI. Does DNaseI inactivated? If so why there is no band in RT reaction?

#2 daixiaol

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Posted 25 March 2002 - 01:56 AM

it is genomic DNA contamination.
DNase cant get rid of all the genomic DNA.  that's i just knew from an expert.  hope it help.




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