Just would like to check if anyone here has done deletion PCRs before.
I am interested in making an inactive Mdm2 (the protein that binds to p53) and I am thinking of using deletion PCR to make an inactive PCR. As this is my first time trying out this technique, I need some guidelines on how to go about doing it. It would be good to discuss this topic here with you all. The Mdm2 is in a plasmid and I am not too sure if it is alright to just use Taq polymerase (I have been using Taq from Bioline all the while for all my PCRs) or is there a need for me to use a high fidelity polymerase such as Pfu?
Edited by jiajia1987, 08 June 2009 - 12:55 AM.