hello,
I have a tube of forward and reverse primer at 100uM concentration, in volume of 50ul.
I need to get both primers to to 50nmol in 100ul final.
How do I do this?
0
1 reply to this topic
#2
Dr Teeth
-
- Active Members
-
- 221 posts
Veteran
1
Neutral
Neutral
Posted 08 June 2009 - 06:43 AM
biogirl1230, on Jun 7 2009, 12:45 PM, said:
hello,
I have a tube of forward and reverse primer at 100uM concentration, in volume of 50ul.
I need to get both primers to to 50nmol in 100ul final.
How do I do this?
I have a tube of forward and reverse primer at 100uM concentration, in volume of 50ul.
I need to get both primers to to 50nmol in 100ul final.
How do I do this?
You would need 50 nl. It would be best to dilute your primer stock to say 1 uM with a 1:100 dilution and then use 5 ul of this in your 100 ul final reaction.
Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley
