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diluting to final primer concentration


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#1 biogirl1230

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Posted 07 June 2009 - 08:45 AM

hello,
I have a tube of forward and reverse primer at 100uM concentration, in volume of 50ul.

I need to get both primers to to 50nmol in 100ul final.

How do I do this?

#2 Dr Teeth

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Posted 08 June 2009 - 06:43 AM

hello,
I have a tube of forward and reverse primer at 100uM concentration, in volume of 50ul.

I need to get both primers to to 50nmol in 100ul final.

How do I do this?


You would need 50 nl. It would be best to dilute your primer stock to say 1 uM with a 1:100 dilution and then use 5 ul of this in your 100 ul final reaction.

Science is simply common sense at its best that is rigidly accurate in observation and merciless to fallacy in logic.
Thomas Henry Huxley




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