Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Label with 35S -met


  • Please log in to reply
1 reply to this topic

#1 saad

saad

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 49 posts
0
Neutral

Posted 06 June 2009 - 07:53 PM

Hi everyone

As you know we can label the protein of interest with 35S-Met only if it contains a few met residues.
My proteins have 7/(425 aa), 5 /( 250 aa), 8/(330 aa) and 5/( 185 aa) met residues respectively,
Are they enough to detect easily by autoradiography?
Is there any rule for these?
Thank you so much in advance.

#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 5,623 posts
388
Excellent

Posted 08 June 2009 - 05:00 PM

You should be able to detect them, usually 3-5 is enough.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.