I thawed my HepG2 cells transfected with RNAi. i take them out of liquid nitrogen and put them water bath at 37 C to complete thawing. Then i put 10 ml of 199 medium with 10% serum. I test the viability with trypan blue staining. i incubated overnight. Today i see my cells have been growing but the can't attach in the flasks, they are in suspension.
What can i do??? anyone can help me??? any substance to help cells out adhering in flask?
P.S. i freezed these cells with Dmem medium with 20% Serum and 10% DMSO. overnight in -20C, then overnight to -80 and then liquid nitrogen.
Edited by Reis.V.P, 06 June 2009 - 01:16 PM.