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Can RIPA buffer be frozen
2 replies to this topic
Posted 05 June 2009 - 09:06 AM
Most of the components in RIPA buffer would be stable at -20 deg C (salts, tris, etc), however, if your RIPA buffer contains protease or phosphatase inhibitors, these will not be stable for long-term storage. We store RIPA buffer containing these for a max of 3-4 wks at -20 deg C. We do not use RIPA buffer with protease/phosphatase inhibitors if it has been stored for more than 1 month.