Posted 05 June 2009 - 07:05 AM
I have 2 cell lines- MDCK T23 and mouse CCD that I have been trying to infect with different three adenoviruses; PIP5K1alpha, PIP5K1beta, and PIP5K1gamma. Strangely the PIP5K1alpha only infects the CCD and not the MDCK. The PIP5K1beta and gamma only infect the MDCK and not the CCD. I have repeated the infections side by side under the same conditions and I consistantly get the same result. I also recently tried infected HeLa cells and only the PIP5K1alpha virus infects. Any suggestions? Why might this be happening? What should I do next?