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protein overexpression question.


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4 replies to this topic

#1 dc11

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Posted 04 June 2009 - 11:58 AM

Hi all,

If a specific protein is strongly expressed in a cell type, can I still overexpress this protein in the cell using a plasmid transient transfection? Thanks a lot in advance for your input.

dc11

#2 bob1

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Posted 04 June 2009 - 05:19 PM

You should be able to as the plasmids typically run off a viral, constitutively expressed promoter.

#3 Bomber

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Posted 05 June 2009 - 12:29 AM

You can, but it might end up in having no apparant effect on the cell.
In case you haven't done it yet, you might want to repress the expression and then check for phenotypes.

#4 warsel

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Posted 06 June 2009 - 02:21 AM

I don't know of any case where that isn't possible. Even if your protein is already highly expressed, a constitutive powerful promoter (like CMV for instance) should be able to boost expression.

If you will see a phenotypic difference though is a different matter (and more so if your phenotypic difference is due to overexpression of ANY protein* or due to specific effects of the protein you chose to overexpress)

*like accumulation of misfolded proteins, putting stress on the protein synthesis machinery, aggregation etc.

Edited by warsel, 06 June 2009 - 02:21 AM.


#5 giota22

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Posted 11 April 2010 - 12:43 PM

Hello, I'm new to this forum. Could you analyze to me what protein overexpression is, where it is used and everything relevant to this subject. Thank you!




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