protein overexpression question.
Posted 04 June 2009 - 11:58 AM
If a specific protein is strongly expressed in a cell type, can I still overexpress this protein in the cell using a plasmid transient transfection? Thanks a lot in advance for your input.
Posted 04 June 2009 - 05:19 PM
Posted 05 June 2009 - 12:29 AM
In case you haven't done it yet, you might want to repress the expression and then check for phenotypes.
Posted 06 June 2009 - 02:21 AM
If you will see a phenotypic difference though is a different matter (and more so if your phenotypic difference is due to overexpression of ANY protein* or due to specific effects of the protein you chose to overexpress)
*like accumulation of misfolded proteins, putting stress on the protein synthesis machinery, aggregation etc.
Edited by warsel, 06 June 2009 - 02:21 AM.
Posted 11 April 2010 - 12:43 PM