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wash buffer for columns


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#1 cycle

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Posted 04 June 2009 - 03:50 AM

Hello,

were running out of wash buffer for spin columns. The manufacturer seems not to be able to provide some extra stuff.

Any ideas how to make the buffer myself? It is the buffer to which you always have to add ethanol before use. I guess its pretty much the same for any spin column kit. Thanks! Cycle

#2 phage434

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Posted 05 June 2009 - 03:19 PM

The Qiagen PE wash buffer is:

* 10 mM Tris-HCl pH 7.5
* 80% ethanol

See http://openwetware.o...prep/Qiagen_kit

#3 cycle

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Posted 07 June 2009 - 10:23 PM

THANKS! Ill try and tell what happens,

cycle

#4 jiajia1987

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Posted 07 June 2009 - 10:41 PM

This is a seriously great link!




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