I used to perform Trizol RNA extraction well. nice pellet good ration and good volume. using fibroblast monolayer cell culture.
lately i started trying RNA extraction from chondrocytes and fibroblasts in high density 3D culture . but after precipitation with isopropyle i am getting gel like pellet. or as if it is two aquous phase one is very small amount around the pellet. i continued with RNA quantifiacation and as expected is not a good ration or volume
any idea why is this hapening . or what should i do ..
i got few normal white pellet in some samples.
i a, checking some troubleshooting and this problem seems to be called as liquid pellet!!!!
Edited by abohollolo, 04 June 2009 - 01:14 AM.