I´m doing some kvantitative analysis of DNA fragments amplified by PCR using a AmpliTaq polymerase.
The analysis is done on a ABI machine but do to a 43 x TC repeat several shadow bands are produced, which make it difficult to kvantify the product.
How can I avoid this??? Is there another polymerase I can use in stead or can I change the PCR programe???
Slippage of PCR polymerase
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