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Drosophila primary neuron preparation

Started by kingsupercat, Jun 04 2009 12:18 AM

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#1 kingsupercat

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Posted 04 June 2009 - 12:18 AM

Does anyone who has experience in handling of primary neuron of fly could provide me detail protocol? I also wonder what reagent you're using to digest the brains? What I'm using now is collagenase but it seems not very powerful...

thanks in advance...

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#2 Carlton H

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Posted 05 June 2009 - 12:26 PM

Try looking here

http://www.pubmedcentral.nih.gov/articlere...i?artid=2556163

...or here...

http://www.jove.com/...ails.stp?ID=200

Hope that helps!
-Carlton

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#3 kingsupercat

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Posted 08 June 2009 - 06:09 PM

Thanks a lot...

Carlton H, on Jun 6 2009, 04:26 AM, said:

Try looking here

http://www.pubmedcentral.nih.gov/articlere...i?artid=2556163

...or here...

http://www.jove.com/...ails.stp?ID=200

Hope that helps!
-Carlton

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