dna separation in native PAGE
Posted 03 June 2009 - 10:29 AM
im trying 2 do separation of nucleic acids by native page. i have 2 resolve by restriction digestion products that are having base pair difference of 56bps.but the fragments size is around 2000bp/
i should choose acrylamide concentration according 2 the resolution i want ie 56bp difference 2 b obserevvd in gel or according to the size of the fragments ie 2000bp.
im confused ??can any1 help plzzz
Posted 03 June 2009 - 04:02 PM
Posted 06 June 2009 - 04:11 AM
Posted 06 June 2009 - 09:23 AM
1. Brody, J. R. and S. E. Kern. 2004. Sodium boric acid: a Tris-free, cooler conductive medium for DNA electrophoresis. BioTechniques 36:214-216
2. Brody, J. R., E. S. Calhoun, E. Gallmeier, T. D. Creavalle, and S. E. Kern. 2004. Ultra-fast high-resolution agarose electrophoresis of DNA and RNA using low-molarity conductive media. BioTechniques 37:598-602.
SB gels are very good at resolving small size differences (see especially reference 2).