I am trying to do an ELISA using a fluorochrome conjugated to my secondary antibody for the first time. I seem to be getting no signal. My fluorochrome is Texas Red and I'm using 595nm as the excitation and 615nm as the emission. What kind of ELISA plate should I be using? I think this may be the issue. I used a cell culture plate that was opaque black, for lack of anything better on hand. I appreciate any advice for a beginner.
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