comparison of bisulfite sequencing and base specific cleavage coupled with Mass
Posted 03 June 2009 - 01:12 AM
Posted 03 June 2009 - 04:18 AM
Very hard to say one for the other without more detail on your part.
Posted 03 June 2009 - 09:19 PM
Posted 04 June 2009 - 01:05 AM
just to add, i will have to get my sequencing done commercially while MS is available in our lab and procedures are optimized (though not specifically this base specific cleavage-MS analysis methodology, but still)
i was just wondering that even if both have their pros and cons, which would be feasible, economically specially.
Posted 04 June 2009 - 04:11 AM
There are pro's and cons, the pro's are it's cheaper than sequencing and supposedly "more representive" than traditional sequencing you need to sequence at least 100 clones to achieve similar accuracy to mass spec, but the big con is, you may not be able to meausre methylation on every CG site in your amplicon of interest because some are too big or too small or non-informative fragments overlap with ones that contain CG's potentiall over or under estimating the methylation
Posted 08 June 2009 - 09:48 PM
one more thing, can anybody send me some article etc on the comparison of these two methods, just for the sake of clarity of concepts????