Freezing cells after fix & perm and also a BrdU question
Posted 02 June 2009 - 07:48 AM
This is my 1st post here, although I use protocol-online a lot.
I have two questions:
1) I want to fix, permeabilize and then freeze my cells in order to immunostain them in another day. I saw that this is possible by adding 90% methanol and then storing the cells at -20º C. Is this feasible?
2) Furthermore, one of the stainings that I'm doing is BrdU. Is it correct to expose cells to BrdU, fix them, and store in 90% methanol at -20º C?
Posted 08 June 2009 - 06:48 AM
Posted 15 June 2009 - 02:59 AM
I don't want to freeze cells specifically to to BrdU staining. I was wondering if it is correct to incubate the cells with BrdU, fix them and then store them to do the rest of the staining in another day.
Posted 23 July 2009 - 06:47 AM
Posted 04 March 2010 - 08:43 AM