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Drosophila tissue homogenization


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#1 abbas

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Posted 02 June 2009 - 03:57 AM

I'm trying to extract proteins and RNAs from adult whole Drosophilas. At the moment I'm using an IKA ultra turrax blender, which is not a tissue homogenizer per se. I would really appreciate if someone could give advice on the best methods on homogenizing Drosophila tissues. Thanks a lot in advance.

#2 AquaPlasmid

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Posted 03 June 2009 - 06:48 AM

I'm trying to extract proteins and RNAs from adult whole Drosophilas. At the moment I'm using an IKA ultra turrax blender, which is not a tissue homogenizer per se. I would really appreciate if someone could give advice on the best methods on homogenizing Drosophila tissues. Thanks a lot in advance.


Abbas,

I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.

Cheers,
AquaPlasmid

#3 abbas

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Posted 03 June 2009 - 10:59 AM

Abbas,

I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.

Cheers,
AquaPlasmid


Thanks for the reply. I rinsed the blender with 70% ethanol between each homogenization. Is bleach better than using ethanol?

#4 AquaPlasmid

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Posted 03 June 2009 - 12:59 PM

Abbas,

I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.

Cheers,
AquaPlasmid


Thanks for the reply. I rinsed the blender with 70% ethanol between each homogenization. Is bleach better than using ethanol?


Yes, bleach! In my hands only bleach rinse can prevent cross-contamination.




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