I'm trying to extract proteins and RNAs from adult whole Drosophilas. At the moment I'm using an IKA ultra turrax blender, which is not a tissue homogenizer per se. I would really appreciate if someone could give advice on the best methods on homogenizing Drosophila tissues. Thanks a lot in advance.
0
3 replies to this topic
#2
AquaPlasmid
-
- Active Members
-
- 65 posts
Enthusiast
4
Neutral
Neutral
Posted 03 June 2009 - 06:48 AM
abbas, on Jun 2 2009, 05:57 AM, said:
I'm trying to extract proteins and RNAs from adult whole Drosophilas. At the moment I'm using an IKA ultra turrax blender, which is not a tissue homogenizer per se. I would really appreciate if someone could give advice on the best methods on homogenizing Drosophila tissues. Thanks a lot in advance.
Abbas,
I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.
Cheers,
AquaPlasmid
#3
abbas
-
- Active Members
-
- 5 posts
member
0
Neutral
Neutral
Posted 03 June 2009 - 10:59 AM
AquaPlasmid, on Jun 3 2009, 04:48 PM, said:
Abbas,
I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.
Cheers,
AquaPlasmid
I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.
Cheers,
AquaPlasmid
Thanks for the reply. I rinsed the blender with 70% ethanol between each homogenization. Is bleach better than using ethanol?
#4
AquaPlasmid
-
- Active Members
-
- 65 posts
Enthusiast
4
Neutral
Neutral
Posted 03 June 2009 - 12:59 PM
abbas, on Jun 3 2009, 12:59 PM, said:
AquaPlasmid, on Jun 3 2009, 04:48 PM, said:
Abbas,
I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.
Cheers,
AquaPlasmid
I wish to be helpful and would like to let you know that AquaRNA extracts both proteins and RNAs from the same specimen and unlike using other methods you don't have a hard time to solubilize the final protein pellet for Western. As far as homogenization goes, I think your turrax would work, and you could use disposible bits or rinse the bits with bleech between each fly. We give out 1-ml trial samples if asked.
Cheers,
AquaPlasmid
Thanks for the reply. I rinsed the blender with 70% ethanol between each homogenization. Is bleach better than using ethanol?
Yes, bleach! In my hands only bleach rinse can prevent cross-contamination.
