I use only 1% of the pull-down products for western, I can see the band. But the protein can not be detected by Blue staining, on the contrary, I saw many other bands in the blue staining. It is wired to me because even the sensitivity between these two methods have 100 folds diffrence, I should be able to see the bands in blue staining, should I?
Did anyone meet such kind of problem before? I use NP40 buffer as the TALON pull-down buffer.
Edited by laurence.ylao, 02 June 2009 - 04:33 AM.