Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

CHIP- Real Time PCR calculations


  • Please log in to reply
1 reply to this topic

#1 rick2000

rick2000

    member

  • Members
  • Pip
  • 6 posts
0
Neutral

Posted 02 June 2009 - 02:29 AM

Hello again !
I started using real time pcr and I am confused about my results. :P
For example, for one of my genes, I have the following results:
Input : 28.16
Mock : 29.88
Antibody 1: 29.25
Antibody 2: 27.25

I am doing the following calculations :
Input : 28.16-3.3 = 24.86
Input- mock = 2 (-5.02)
Input- Antibody 1= 2 (-4.39)
Input - Antibody 2= 2(-2.39)
Mock : 3%
Antibody 1: 4,7 %
Antibody 2: 19.2 %

Is something wrong with the calculations? Should I do something else?
Thanks in advance

#2 epi123

epi123

    member

  • Active Members
  • Pip
  • 20 posts
0
Neutral

Posted 21 June 2009 - 08:01 AM

check out ChIP qpCR template at invitrogen. for percent input, it does depend on what percent of input you are removing. for example, if removing 1%, then correction factor is 6.6. or if 10%, then correction factor is 3.3

hope this helps

http://www.invitroge...p-analysis.html




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.