1 reply to this topic

[res_org]

Can any body share their protocol or provide me a link to read how to estimate transgene copy numebr?

I have never done it but understand that it can be done by southern blot or real time PCR. Real time PCR seems to be less cumbersome.

I have never done real time PCR as well but use PCR very often in my project.

Thanks for your help!

[zodiac1505]

res_org, on Jun 1 2009, 11:20 PM, said:

Can any body share their protocol or provide me a link to read how to estimate transgene copy numebr?

I have never done it but understand that it can be done by southern blot or real time PCR. Real time PCR seems to be less cumbersome.

I have never done real time PCR as well but use PCR very often in my project.

Thanks for your help!

I have been trying to get my Southern blot to work for a while So I looked up the RT- PCR way and found this link. Maybe you can look through it. I am not planning to buy the kit, but to use my GFP primers. Maybe you could work out something similar

http://catalog.takara-bio.co.jp/en/product...itid=U100006070

Edited by zodiac1505, 05 July 2010 - 07:12 AM.