Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

transgene copy number


  • Please log in to reply
1 reply to this topic

#1 res_org

res_org

    member

  • Active Members
  • Pip
  • 5 posts
0
Neutral

Posted 01 June 2009 - 01:20 PM

Can any body share their protocol or provide me a link to read how to estimate transgene copy numebr?

I have never done it but understand that it can be done by southern blot or real time PCR. Real time PCR seems to be less cumbersome.

I have never done real time PCR as well but use PCR very often in my project.

Thanks for your help!

#2 zodiac1505

zodiac1505

    Enthusiast

  • Moderators
  • PipPipPipPipPip
  • 92 posts
3
Neutral

Posted 05 July 2010 - 07:12 AM

Can any body share their protocol or provide me a link to read how to estimate transgene copy numebr?

I have never done it but understand that it can be done by southern blot or real time PCR. Real time PCR seems to be less cumbersome.

I have never done real time PCR as well but use PCR very often in my project.

Thanks for your help!



I have been trying to get my Southern blot to work for a while :lol: So I looked up the RT- PCR way and found this link. Maybe you can look through it. I am not planning to buy the kit, but to use my GFP primers. Maybe you could work out something similar ;)

http://catalog.takar...itid=U100006070

Edited by zodiac1505, 05 July 2010 - 07:12 AM.





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.