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transient expression

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#1 aztecan princess

aztecan princess


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Posted 01 June 2009 - 12:22 PM

Doing transient expression transfecting a plasmid in HEK293:
What is the best time after transfection to do RNA extraction and RT-PCR to detect the mRNA of the gen in the plasmid?

And what is the minimum time?? Do you think after 24 h I could detect easily the mRNA???

Thanks in advantage!!!

#2 pcrman



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Posted 01 June 2009 - 06:05 PM

I would say the best time frame is around 48 hrs. The minimum time is less than 24 hrs. This can be told by transfecting a GFP plasmid.

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