Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

transient expression


  • Please log in to reply
1 reply to this topic

#1 aztecan princess

aztecan princess

    member

  • Active Members
  • Pip
  • 20 posts
0
Neutral

Posted 01 June 2009 - 12:22 PM

Doing transient expression transfecting a plasmid in HEK293:
What is the best time after transfection to do RNA extraction and RT-PCR to detect the mRNA of the gen in the plasmid?

And what is the minimum time?? Do you think after 24 h I could detect easily the mRNA???

Thanks in advantage!!!

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
68
Excellent

Posted 01 June 2009 - 06:05 PM

I would say the best time frame is around 48 hrs. The minimum time is less than 24 hrs. This can be told by transfecting a GFP plasmid.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.