Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

blunt ligation


  • Please log in to reply
3 replies to this topic

#1 deespike

deespike

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 66 posts
0
Neutral

Posted 01 June 2009 - 02:39 AM

hi all
im working on blunt end ligation .i have searched this forum for some tips on this ligation but im confused with the temperature usage for the reaction.what is the optimum temoerature for performing blunt end liagtion?

thanks in advance

#2 WHR

WHR

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 49 posts
0
Neutral

Posted 01 June 2009 - 03:11 AM

hi all
im working on blunt end ligation .i have searched this forum for some tips on this ligation but im confused with the temperature usage for the reaction.what is the optimum temoerature for performing blunt end liagtion?

thanks in advance


T4 ligase is most active at 37 degree C. I usually perform ligation reaction at RT for convenience.

Edited by WHR, 01 June 2009 - 06:48 AM.


#3 madrius1

madrius1

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 114 posts
0
Neutral

Posted 01 June 2009 - 05:48 AM

People sometimes do it at 16 C O/N. Really depends on your needs/time!

#4 T C

T C

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 277 posts
0
Neutral

Posted 01 June 2009 - 08:19 PM

Hey,

Read this too:

http://www.protocol-...re for ligation

Best,
TC

hi all
im working on blunt end ligation .i have searched this forum for some tips on this ligation but im confused with the temperature usage for the reaction.what is the optimum temoerature for performing blunt end liagtion?

thanks in advance






Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.