Chosing the best housekeeping gene!
Posted 31 May 2009 - 10:00 PM
I'm trying to detect the level of expression for two different mRNA with real-time PCR (SYBR green) during apoptosis. I'm using GAPDH and b-actin as my control.
But I need to change both my housekeeping genes primers as they start to show downregulation during apoptosis.
I'm looking for a substitute for these two genes. Do you think 18S would stay constant during apoptosis?
Posted 02 June 2009 - 04:32 PM
Posted 02 June 2009 - 05:36 PM
Posted 02 June 2009 - 08:10 PM
so I'm gonna design new primers for 18s, B2M and HPRT
Posted 04 June 2009 - 01:26 AM
Posted 16 June 2009 - 06:05 AM