Hi all,
i'm trying to clone two genes to pENTR D TOPO vector, but all I got were negative clones. The size is 2.2kb and 7kb. 50 ug/ml Kan was used. Any suggestion will be appricated. Thanks.
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Posted 31 May 2009 - 08:52 PM
Hey,
You have to give more details for anyone to figure out whats wrong.
Best,
TC
You have to give more details for anyone to figure out whats wrong.
Best,
TC
felicity, on Jun 1 2009, 12:40 AM, said:
Hi all,
i'm trying to clone two genes to pENTR D TOPO vector, but all I got were negative clones. The size is 2.2kb and 7kb. 50 ug/ml Kan was used. Any suggestion will be appricated. Thanks.
i'm trying to clone two genes to pENTR D TOPO vector, but all I got were negative clones. The size is 2.2kb and 7kb. 50 ug/ml Kan was used. Any suggestion will be appricated. Thanks.
