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pENTR TOPO cloning


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#1 felicity

felicity

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Posted 31 May 2009 - 11:10 AM

Hi all,

i'm trying to clone two genes to pENTR D TOPO vector, but all I got were negative clones. The size is 2.2kb and 7kb. 50 ug/ml Kan was used. Any suggestion will be appricated. Thanks.

#2 T C

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Posted 31 May 2009 - 08:52 PM

Hey,

You have to give more details for anyone to figure out whats wrong.

Best,
TC

Hi all,

i'm trying to clone two genes to pENTR D TOPO vector, but all I got were negative clones. The size is 2.2kb and 7kb. 50 ug/ml Kan was used. Any suggestion will be appricated. Thanks.






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