Neuro2A and Neuroblastoma B104- how to culture?
Posted 31 May 2009 - 04:11 AM
Posted 01 June 2009 - 02:08 AM
Harvest confluent cultures using trypsin but remember not to over split. Would suggest 1:2 - 1:3 for neuro2A. Can split B104 using higher ratios (eg 1:5).
Hope this helps.
Posted 07 June 2009 - 01:45 AM
DMEM plus 10% (5% also works well) serum is all you need for B104 cells. these cells grow even if you don't want them to grow... don't worry, they are easy to culture. in my experience, only the number of splits (propagations) can lead to a bad growth (do not exceed over 15), anyway, it depends on the individual crio-preservation cell clone conditions.