DMSO and Serum
Posted 30 May 2009 - 05:29 AM
Can anyone help me with this doubts.....
1) how DMSO works in cell freezing? what about Glicerol?
2) increasing or lowing FBS drastically can be damaged to the cells? for exemple cells used to grow in 10% FBS and then i replace with a media 25% FBS.
3)what is the best way of thaw FBS? i have seen in the botton of Falcon tube, something like "gelatin"..... protein precipitation???
Posted 01 June 2009 - 04:45 PM
Edited by bob1, 01 June 2009 - 04:47 PM.