Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Lyophilize


  • Please log in to reply
5 replies to this topic

#1 shimshady

shimshady

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 37 posts
0
Neutral

Posted 29 May 2009 - 07:52 AM

How do you guys do it?

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,619 posts
118
Excellent

Posted 29 May 2009 - 09:58 AM

do you have the apparatus?

what volume are you talking about?
talent does what it can
genius does what it must
i do what i get paid to do

#3 shimshady

shimshady

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 37 posts
0
Neutral

Posted 29 May 2009 - 10:20 AM

no real apparatus but im talking like 30-60 mL. However we do have everything to do it without one, im more just concerned with the temp at which you do it, any suggestions how to freeze it. Do you keep it frozen the whole time? THings like that.

Edited by shimshady, 29 May 2009 - 10:34 AM.


#4 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,619 posts
118
Excellent

Posted 03 June 2009 - 06:49 AM

we would shell freeze the solution, in a round flask, using a dry ice/acetone bath to freeze the solution. the same dry ice/acetone bath would be placed between the vacuum pump and the sample as a condensing vessel.

you pull a strong vacuum and let it go. the frozen sample will not defrost as long as it doesn't touch a (warm) surface.

when it is fully dry, scrape the powder out of the flask and store it in a vial or you can just seal the flask and store.

i think i covered just about everything (i haven't done this for a long time so forgive me if i left anything out), if not then ask.
talent does what it can
genius does what it must
i do what i get paid to do

#5 shimshady

shimshady

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 37 posts
0
Neutral

Posted 07 June 2009 - 12:07 PM

That is exactly what i was looking for. Thanks. My next question is about how long does it take? i know it differs depending on volume but do you let it go overnight? Im looking at aorund 30-60 mL.

#6 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,619 posts
118
Excellent

Posted 08 June 2009 - 06:51 AM

if memory serves me correctly (it's been many, many moons since i did this), we mostly let it go overnight. we occasionally completed it during the day (if we started early enough). sometimes it had to go more than overnight.

time to dry is very dependent upon the strength of the vacuum that you pull as well as the starting volume.
talent does what it can
genius does what it must
i do what i get paid to do




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.