Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

to increase the sensitivity of SDS-PAGE silver staining??


  • Please log in to reply
1 reply to this topic

#1 rick112

rick112

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 71 posts
0
Neutral

Posted 28 May 2009 - 10:31 PM

hi

Do any one is familiar with methods to improve sensitivity of silver staining technique used for SDS-PAGE??

thanks a lot

#2 mdfenko

mdfenko

    an elder

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 2,785 posts
132
Excellent

Posted 29 May 2009 - 05:55 AM

one thing that we have done is stain twice (with or, mostly, without a destaining step). but the background can get very dark.

with the silver stain method we use (modified, by us, merril) we still get a translucent (amber) background.

Edited by mdfenko, 29 May 2009 - 05:55 AM.

talent does what it can
genius does what it must
i do what i get paid to do




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.