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to increase the sensitivity of SDS-PAGE silver staining??

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#1 rick112



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Posted 28 May 2009 - 10:31 PM


Do any one is familiar with methods to improve sensitivity of silver staining technique used for SDS-PAGE??

thanks a lot

#2 mdfenko


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Posted 29 May 2009 - 05:55 AM

one thing that we have done is stain twice (with or, mostly, without a destaining step). but the background can get very dark.

with the silver stain method we use (modified, by us, merril) we still get a translucent (amber) background.

Edited by mdfenko, 29 May 2009 - 05:55 AM.

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