hi
Do any one is familiar with methods to improve sensitivity of silver staining technique used for SDS-PAGE??
thanks a lot
to increase the sensitivity of SDS-PAGE silver staining??
Started by rick112, May 28 2009 10:31 PM
1 reply to this topic
#1
Posted 28 May 2009 - 10:31 PM
#2
Posted 29 May 2009 - 05:55 AM
one thing that we have done is stain twice (with or, mostly, without a destaining step). but the background can get very dark.
with the silver stain method we use (modified, by us, merril) we still get a translucent (amber) background.
with the silver stain method we use (modified, by us, merril) we still get a translucent (amber) background.
Edited by mdfenko, 29 May 2009 - 05:55 AM.
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