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Neuro2A and Neuroblastoma B104- how to culture?


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3 replies to this topic

#1 mia

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Posted 28 May 2009 - 06:39 AM

Hi, could anyone help me out with information on how to culture the mouse neuroblastoma cell line Neuro 2A and rat neuroblastoma line B104 ? I could not figure out whether a particular coating is needed for those cells. And, does somebody have experience in how those cells grow in RPMI Medium + 10%FCS (instead of the published DMEM +10%FCS)? Thanks.

#2 Pyaria

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Posted 05 June 2009 - 10:58 PM

I've cultured Neuro2A cells before and I just used the normal tissue culture plates. I found that extra plating or treatment was not necessary beforehand.

No idea how they grow in RPM media though.

#3 BioSaint

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Posted 26 July 2010 - 03:55 AM

Add some NAA (non-essential amino acids, Gibco) That ll help to grow them with RPMI medium.

#4 Tong

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Posted 22 July 2011 - 11:11 AM

For neuro 2a, I've cultured by using media which are EMEM/EBSS+10% FBS+1%Penstrp+1%L-glutamine




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