Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Re: Low phosphate media forBacillus/Bacillus transformation protocol


  • Please log in to reply
1 reply to this topic

#1 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 29 March 2001 - 10:00 PM

Hello,It depends which kind of protein you want to extract.I wash the pieces of tissue in PBS ice cold, centrifuge at 4C 3400rpm for 10', then omogenize(very important!) better if with polytron in Suspension Buffer (see Maniatis) or Lysis buffer (no Triton x-100 inside!), boil for 10' and centrifuge at 10,000g for 10'. I use an aliquot of the extract for Bradford assay and then I put the 2x SDS loading buffer (1:1).I hope this could be useful. For every more questions you can write to me.Good Luck!Monica

#2 anonymous

anonymous

    Veteran

  • PipPipPipPipPipPipPipPipPipPip
  • 1,890 posts
1
Neutral

Posted 29 March 2001 - 10:00 PM

does anyone have a general protein extraction protocol for tissue? i have one for cells but not from whole tissue. i want to run the samples on a western. thanks




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.