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[shicaeon]

Dear all,

I am going to work on RNase H digestion method. I want to remove a number of of gene-specific RNA from a pool of total RNA extracted. I plan to design oligos that are complementary to those RNA targets. And through the RNA:DNA hybrid formed, RNase H digestion can be used to remove thr RNA targets. Since I am new to RNase H digestion, I would like to ask what purity of oligos should I used? Can I simply used desalted ones? As far as I know, desalted oligos may contains truncated ones, but I think as long as the RNA:DNA hybrid are formed, even with the truncated ones that are in small amount, it shouldn't affect the result, is it?

Thanks in advance!